New insights into the specificity and processivity of two novel pectinases from Verticillium dahliae

Pectin, the major non-cellulosic component of primary cell wall can be degraded by polygalacturonases (PGs) and pectin methylesterases (PMEs) during pathogen attack on plants. We characterized two novel enzymes, VdPG2 VdPME1, from fungal plant Verticillium dahliae. VdPME1 was most active citrus methylesterified (55–70%) at pH 6 a temperature 40 °C, while polygalacturonic acid 5 50 °C. Using LC-MS/MS oligoprofiling, various pectins, mode action were determined. shown to processive, in accordance with electrostatic potential enzyme. identified as endo-PG releasing both non-methylesterified oligogalacturonides (OGs). Additionally, when flax roots used substrate, acetylated OGs detected. The comparisons released Verticillium-susceptible partially resistant cultivars new possible elicitor defence responses.